通过给予过量卵清蛋白产生的非沉淀性豚鼠抗体。
Non-precipitating guinea-pig antibodies produced by administration of excessive doses of ovalbumin.
作者信息
Tamoto K, Nakamura T, Koyama J
出版信息
Immunology. 1974 Mar;26(3):455-63.
Partial paralysis of the antibody response in guinea-pigs produced by administration of an excessive dose of ovalbumin was accompanied by the formation of non-precipitating IgG1 and IgG2 antibodies. Immunochemical properties of these non-precipitating antibodies were compared with those of the precipitating antibodies obtained from animals given a normal dose of ovalbumin. Non-precipitating IgG2 antibodies did not fix complement and non-precipitating IgG1 antibodies elicited PCA but with less efficiency than precipitating IgG1 antibodies. On the other hand, the ammonium sulphate globulin precipitation technique for the determination of antibody affinity revealed that precipitating and non-precipitating antibodies were similar with regard to valency and average affinity for the antigen. These results are compatible with the hypothesis that non-precipitating antibodies result from a limited recognition of multiple antigenic determinants on ovalbumin molecules.
给豚鼠过量注射卵清蛋白导致抗体反应部分麻痹，同时伴有非沉淀性IgG1和IgG2抗体的形成。将这些非沉淀性抗体的免疫化学特性与给正常剂量卵清蛋白的动物所获得的沉淀性抗体的特性进行了比较。非沉淀性IgG2抗体不固定补体，非沉淀性IgG1抗体引发被动皮肤过敏反应（PCA），但其效率低于沉淀性IgG1抗体。另一方面，用于测定抗体亲和力的硫酸铵球蛋白沉淀技术表明，沉淀性抗体和非沉淀性抗体在对抗原的价数和平均亲和力方面相似。这些结果与以下假设相符，即非沉淀性抗体是由于对卵清蛋白分子上多个抗原决定簇的有限识别所致。
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