Ganguly U, Greenough W B
Infect Immun. 1975 Feb;11(2):343-9. doi: 10.1128/iai.11.2.343-349.1975.
The extracellular concentration of cyclic adenosine 3',5'-monophosphate (AMP) of three different strains of Vibrio cholerae growing in syncase medium were measured. Cyclic AMP secreted by V. cholerae 569B varied widely, with different carbon sources. Mutant 13, which produced little or no toxin, released half the amount of cyclic AMP as the wild type. The release of less cyclic AMP into the medium by mutant 13 may be accounted for by the lower activity of adenylate cyclase observed. High glucose (3%) in the culture medium reduced the concentration of cyclic AMP both in wild type and mutant 13. Reduction of cyclic AMP levels at high concentrations of glucose (3%) occurred without change of adenylate cyclase activity. The release of enterotoxin to the medium varied with carbon sources but was independent of conditions which reduced the cyclic AMP both within the cell and the medium. Neither adenylate cyclase activity nor toxin production was reduced by an increase concentration of glucose in wild-type V. cholerae, whereas cyclic AMP levels were reduced by sixfold. A lower activity of the adenylate cyclase was observed in a mutant of V. cholerae which produced no detectable toxin. Thus, a correlation exists between toxin production and adenylate cyclase activity in V. cholerae.
对在同步酶培养基中生长的三种不同霍乱弧菌菌株的细胞外3',5'-环磷酸腺苷（cAMP）浓度进行了测定。霍乱弧菌569B分泌的cAMP因碳源不同而有很大差异。几乎不产生毒素或不产生毒素的突变体13释放的cAMP量仅为野生型的一半。突变体13向培养基中释放的cAMP较少，可能是由于观察到的腺苷酸环化酶活性较低。培养基中高浓度葡萄糖（3%）会降低野生型和突变体13中的cAMP浓度。在高浓度葡萄糖（3%）下cAMP水平降低，但腺苷酸环化酶活性未发生变化。向培养基中释放的肠毒素因碳源而异，但与降低细胞内和培养基中cAMP的条件无关。在野生型霍乱弧菌中，葡萄糖浓度升高既不会降低腺苷酸环化酶活性，也不会减少毒素产生，而cAMP水平却降低了六倍。在一株检测不到毒素产生的霍乱弧菌突变体中，观察到腺苷酸环化酶活性较低。因此，霍乱弧菌中毒素产生与腺苷酸环化酶活性之间存在相关性。
