Eriksson K O, Palm A, Hjertén S
Department of Biochemistry, University of Uppsala, Biomedical Center, Sweden.
Anal Biochem. 1992 Mar;201(2):211-5. doi: 10.1016/0003-2697(92)90330-a.
A micropreparative capillary electrophoresis apparatus equipped with a new type of fraction collection device is described: solutes, such as proteins, are adsorbed onto a moving blotting membrane (for instance a polyvinylidene difluoride membrane) as they migrate electrophoretically out of the capillary. The adsorbed proteins are visualized by conventional protein staining methods or by fluorescent labeling. Specific identification of separated components by an immunological technique is demonstrated. The method also offers the potential to analyze proteins and peptides collected on the membrane by gas phase sequencing and mass spectrometry.
溶质（如蛋白质）在通过电泳从毛细管中迁移出来时，会吸附到移动的印迹膜（例如聚偏二氟乙烯膜）上。吸附的蛋白质通过常规蛋白质染色方法或荧光标记进行可视化。展示了通过免疫技术对分离成分进行特异性鉴定。该方法还提供了通过气相测序和质谱分析膜上收集的蛋白质和肽的潜力。
