Zhang Shiwu, Guo Hua, Zhang Danfang, Zhang Wenzhi, Zhao Xiulan, Ren Zhiyong, Sun Baocun
Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, PR China.
Oncol Rep. 2006 Jan;15(1):15-20.
Several microcirculation patterns in tumors have been reported, including vasculogenic mimicry (VM), mosaic vessels (MV) and endothelium-dependent vessels. To investigate the sources of blood supply during different tumor stages, we studied the correlation between expression of vasculogenic mimicry (VM), mosaic vessels (MV) and endothelium-dependent vessels in a mouse melanoma xenograph. Sixty C57 mice were divided into 12 groups (5 mice per group) and inoculated with B16 melanoma cells. Eleven days later, the average tumor size was approximately 0.2 to 0.3 cm. From days 11 to 22, one group per day was randomly sacrificed, and the density of vasculogenic mimicry, mosaic vessels and endothelium-dependent vessels was measured in tumor tissue sections. Immunohistochemical dual-staining and electronic microscopy were also used to confirm the vessel types. All three types of microcirculation patterns were observed during tumor development. In the early stage of tumor growth, vasculogenic mimicry is the main pattern of blood supply. As the area of tumor tissue expands and the number of endothelium increase, vasculogenic mimicry is replaced by endothelium-dependent vessels. Mosaic vessels might be the interim state between vasculogenic mimicry and endothelium-dependent vessels. The number of endothelium-dependent vessels correlated with the size of the tumor (r=0.718, P=0.009), while the number of vasculogenic mimicry was inversely correlated (r=0.77, P=0.003). In conclusion, the number of vasculogenic mimicry decreased and the number of endothelial-dependent vessels increased during tumor growth.
已有报道称肿瘤存在多种微循环模式，包括血管生成拟态（VM）、镶嵌血管（MV）和内皮依赖性血管。为了研究不同肿瘤阶段的血液供应来源，我们在小鼠黑色素瘤异种移植模型中研究了血管生成拟态（VM）、镶嵌血管（MV）和内皮依赖性血管表达之间的相关性。将60只C57小鼠分为12组（每组5只），接种B16黑色素瘤细胞。11天后，肿瘤平均大小约为0.2至0.3厘米。从第11天到第22天，每天随机处死一组，测量肿瘤组织切片中血管生成拟态、镶嵌血管和内皮依赖性血管的密度。还使用免疫组织化学双重染色和电子显微镜来确认血管类型。在肿瘤发展过程中观察到了所有三种微循环模式。在肿瘤生长早期，血管生成拟态是主要的血液供应模式。随着肿瘤组织面积扩大和内皮数量增加，血管生成拟态被内皮依赖性血管所取代。镶嵌血管可能是血管生成拟态和内皮依赖性血管之间的过渡状态。内皮依赖性血管的数量与肿瘤大小相关（r = 0.718，P = 0.009），而血管生成拟态的数量呈负相关（r = 0.77，P = 0.003）。总之，在肿瘤生长过程中，血管生成拟态的数量减少，内皮依赖性血管的数量增加。
