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中国福建某稻田中蓝藻群落的生物多样性与季节变化

Biodiversity and seasonal variation of the cyanobacterial assemblage in a rice paddy field in Fujian, China.

作者信息

Song Tieying, Mårtensson Lotta, Eriksson Torsten, Zheng Weiwen, Rasmussen Ulla

机构信息

Biotechnology Center, Fujian Academy of Agricultural Sciences, Fuzhou, PR China.

出版信息

FEMS Microbiol Ecol. 2005 Sep 1;54(1):131-40. doi: 10.1016/j.femsec.2005.03.008.

Abstract

Cyanobacteria are one of the main components of the microbiota in rice paddy fields and significantly contribute to its fertilization. The diversity and changes of the cyanobacterial assemblage were investigated during a rice growth season and after harvest in a paddy field located in Fujian Province, China. The cyanobacterial populations were analyzed by a semi-nested PCR, followed by denaturing gradient gel electrophoresis analysis. Twenty-four phylotypes were identified from the denaturing gradient gel electrophoresis profiles. The number of cyanobacterial phylotypes showed a seasonal variation and reached a peak in September, both in the upper (0-5 cm) and the deeper (10-15 cm) soil fractions. Some cyanobacterial sequences were only present during the rice growth season, while others were only found after harvest.

摘要

蓝细菌是稻田微生物群的主要组成部分之一,对稻田施肥有重要贡献。在中国福建省的一块稻田中,研究了水稻生长季节和收获后蓝细菌群落的多样性及变化。通过半巢式PCR分析蓝细菌种群,随后进行变性梯度凝胶电泳分析。从变性梯度凝胶电泳图谱中鉴定出24个系统型。蓝细菌系统型的数量呈现季节性变化,9月在上层(0 - 5厘米)和深层(10 - 15厘米)土壤部分均达到峰值。一些蓝细菌序列仅在水稻生长季节出现,而其他序列仅在收获后被发现。

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