Dolan M L, Coetzee M L, Spangler M, Ove P
J Natl Cancer Inst. 1975 Jan;54(1):163-9. doi: 10.1093/jnci/54.1.163.
The effect of a serum factor purified from normal rat serum on growth and incorporation of 3-H-thymidine and 3-H-leucine was investigated with the use of 3T3 and L cells. The factor was not present in serum of hepatoma-bearing animals at a time when the weight of the hepatomas was equal to or greater than liver weight. The factor disappeared gradually from the serum of hepatoma-bearing animals, and the disappearance was proportional to the increase in the size of the hepatomas. Diminished amounts of the factor could be detected as early as 10 days after hepatoma transplant, and the factor was absent at 30 days after hepatoma transplant from the serum of rats bearing hepatoma 7777. Medium supplemented with 5% normal rat serum supported incorporation of lavel into 3T3 and L cells and growth of these cells, as did medium supplemented with 10% calf serum. Serum from hepatoma-bearing animals was only approximately equal to 50% as efficient as normal rat serum in supporting growth and label incorporation. The addition of purified factor to the serum from hepatoma-bearing animals restored the ability of the serum to support growth and label incorporation to between 70 and 90% of that found with normal rat serum as a medium supplement. The factor also enhanced incorporation of 3-H-thymidine following release of 3T3 cells from contact inhibition.
利用3T3细胞和L细胞研究了从正常大鼠血清中纯化的一种血清因子对3 - H - 胸腺嘧啶核苷和3 - H - 亮氨酸掺入及细胞生长的影响。当肝癌动物的肝癌重量等于或超过肝脏重量时，其血清中不存在该因子。该因子在肝癌动物血清中逐渐消失，且其消失与肝癌大小的增加成比例。早在肝癌移植后10天就能检测到该因子的量减少，而在移植肝癌7777的大鼠血清中，肝癌移植后30天该因子就不存在了。补充5%正常大鼠血清的培养基支持3T3细胞和L细胞掺入放射性标记及这些细胞的生长，补充10%小牛血清的培养基也有同样效果。肝癌动物血清在支持细胞生长和标记掺入方面的效率仅约为正常大鼠血清的50%。向肝癌动物血清中添加纯化因子可使血清支持生长和标记掺入的能力恢复到作为培养基补充物时正常大鼠血清的70%至90%。该因子还能增强3T3细胞从接触抑制中释放后3 - H - 胸腺嘧啶核苷的掺入。
