Pinheiro A M, Costa S L, Freire S M, Meyer R, Almeida M A O, Tardy M, El Bachá R, Costa M F D
Laboratório de Neuroquímica e Biologia Celular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Brazil.
Exp Parasitol. 2006 Mar;112(3):193-7. doi: 10.1016/j.exppara.2005.10.008. Epub 2005 Dec 5.
The effect of Neospora caninum, a parasite that causes abortion and neuromuscular changes, has been investigated on a major population of neural cells, the astrocytes. Highly enriched astroglial primary cultures obtained from neonatal rats were infected after 21 days of culture. Astroglial reactivity, IL-10 and IFN-gamma expression, and cell viability (lactate dehydrogenase activity, metabolization of tetrazolium salt, and trypan blue exclusion assay) have been investigated after 24 and 72 h of infection. Astroglial hypertrophy, gliofilament reorganization, metabolic changes suggesting hypoxia and a strong IL-10 release have been observed in the infected cells. These results show that neural cells are targets for the parasite and that astrocytes may contribute to the CNS immune response to the parasite.
犬新孢子虫是一种可导致流产和神经肌肉变化的寄生虫，其对主要神经细胞群——星形胶质细胞的影响已得到研究。从新生大鼠获取的高度富集的星形胶质细胞原代培养物在培养21天后受到感染。在感染24小时和72小时后，对星形胶质细胞反应性、白细胞介素-10（IL-10）和γ干扰素（IFN-γ）表达以及细胞活力（乳酸脱氢酶活性、四氮唑盐代谢和台盼蓝排斥试验）进行了研究。在受感染细胞中观察到星形胶质细胞肥大、胶质丝重组、提示缺氧的代谢变化以及强烈的IL-10释放。这些结果表明神经细胞是该寄生虫的靶标，并且星形胶质细胞可能有助于中枢神经系统对该寄生虫的免疫反应。
