Kawano-Yamamoto Chizuru, Muroi Kazuo, Nagatsuka Yasuko, Higuchi Masato, Kikuchi Satoru, Nagai Tadashi, Hakomori Sen-Itiroh, Ozawa Keiya
Division of Hematology, Department of Medicine, Jichi Medical School, Minamikawachi, Tochigi 329-0498, Japan.
Leuk Res. 2006 Jul;30(7):829-39. doi: 10.1016/j.leukres.2005.10.021. Epub 2005 Dec 5.
A new erythroblastic leukemia cell line (EEB) was established from a patient with early erythroblastic leukemia. The cells had features of immature erythroblasts, including an agranular basophilic cytoplasm and CD36, CD71, CD175s (sialyl-Tn) and CD235a (glycophorin A) expression without CD41 expression, myeloperoxidase activity and platelet-peroxidase activity. The cells were confirmed to be of the erythroid lineage based on expression of the gamma-globin message. They were induced to differentiate into benzidine-positive cells by hemin and delta-amino levulinic acid (delta-ALA). An analysis of cell membrane lipids showed that EEB cells contain a type of glycerolipid, phosphatidylglucose (PhGlc), but not unbranched type 2 chains, i antigens. GL-7 which is a recombinant Fab fragment of GL-2 and binds to PhGlc, induced production of hemoglobin F (HbF) associated with accumulation of the gamma-globin (gamma-globin) message in EEB cells. The GL-7-mediated erythroid differentiation was associated with apoptosis. These results suggest that direct signaling to PhGlc mediates erythroid differentiation and apoptosis in EEB cells.
一种新的成红细胞白血病细胞系（EEB）是从一名早期成红细胞白血病患者体内建立的。这些细胞具有未成熟成红细胞的特征，包括无颗粒的嗜碱性细胞质以及CD36、CD71、CD175s（唾液酸-Tn）和CD235a（血型糖蛋白A）表达，但无CD41表达、髓过氧化物酶活性和血小板过氧化物酶活性。基于γ-珠蛋白信息的表达，证实这些细胞属于红系谱系。它们被血红素和δ-氨基乙酰丙酸（δ-ALA）诱导分化为联苯胺阳性细胞。细胞膜脂质分析表明，EEB细胞含有一种甘油脂质，磷脂酰葡萄糖（PhGlc），但不含无分支的2型链、i抗原。GL-7是GL-2的重组Fab片段，可与PhGlc结合，它在EEB细胞中诱导产生与γ-珠蛋白（γ-globin）信息积累相关的血红蛋白F（HbF）。GL-7介导的红系分化与细胞凋亡相关。这些结果表明，向PhGlc的直接信号传导介导了EEB细胞中的红系分化和细胞凋亡。
