Tzanetakis Ioannis E, Susaimuthu James, Gergerich Rose C, Martin Robert R
Department of Botany and Plant Pathology & Center for Gene Research and Biotechnology, Oregon State University, Corvallis, 97331, USA.
Virus Res. 2006 Mar;116(1-2):196-200. doi: 10.1016/j.virusres.2005.10.003. Epub 2005 Dec 5.
The complete nucleotide sequence of a novel member of the genus Crinivirus (family Closteroviridae), isolated from blackberry and tentatively named Blackberry yellow vein associated virus, was determined. The virus possesses a bipartite genome. RNA 1 is 7,801 nucleotides in length and papain-like protease, methyltransferase, RNA helicase and RNA-dependent RNA polymerase motifs have been identified in the proteins coded for by this molecule. The polymerase is probably expressed via a +1 ribosomal frameshift, a common feature among members of the Closteroviridae. RNA 2 is 7,917 nucleotides long and encodes nine open reading frames, similar in size and position to orthologous genes of other criniviruses with the exception of a second hydrophobic peptide found near the 5' terminus of the molecule. Phylogenetic analysis revealed a close relationship between Blackberry yellow vein associated virus and Beet pseudo yellows virus, another crinivirus that infects small fruit crops.
