Zhang Peng, Han Tao-Tao, Tang Jia-Xuan, Zhong Kui, Ma Yu, Smith William K, Zhao Wei-Guo, Lu Quan-You
College of Biotechnology, Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, Jiangsu, China.
Key Laboratory of Genetic Improvement of Silkworm and Mulberry, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, Jiangsu, China.
Arch Virol. 2023 Jan 7;168(2):50. doi: 10.1007/s00705-022-05657-7.
The whole genome sequence of mulberry crinivirus (MuCV), a novel member of the genus Crinivirus (family Closteroviridae) identified in mulberry (Morus alba L), was determined. The virus possesses a bipartite genome. RNA1 contains 8571 nucleotides (nt) with four open reading frames (ORFs). ORF1a encodes a putative polyprotein with papain-like protease, methyltransferase, and RNA helicase domains. ORF1b putatively encodes an RNA-dependent RNA polymerase (RdRp), which is probably expressed via a + 1 ribosomal frameshift. RNA2 consists of 8082 nt, containing eight ORFs that are similar in size and position to orthologous genes of other criniviruses. Phylogenetic analysis based on RdRp amino acid sequences of criniviruses placed MuCV in group 1.
在桑树（桑属植物）中鉴定出的一种新型毛形病毒属（长线形病毒科）成员——桑毛形病毒（MuCV）的全基因组序列已被测定。该病毒具有双分体基因组。RNA1包含8571个核苷酸（nt），有四个开放阅读框（ORF）。ORF1a编码一个具有类木瓜蛋白酶、甲基转移酶和RNA解旋酶结构域的假定多聚蛋白。ORF1b推测编码一种RNA依赖的RNA聚合酶（RdRp），它可能通过 +1核糖体移码表达。RNA2由8082 nt组成，包含八个ORF，其大小和位置与其他毛形病毒的直系同源基因相似。基于毛形病毒RdRp氨基酸序列的系统发育分析将MuCV归入第1组。
