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对来自中国云南腾冲热海的几种嗜热栖热菌菌株进行的系统发育分析。

Phylogenetic analysis of several Thermus strains from Rehai of Tengchong, Yunnan, China.

作者信息

Lin Lianbing, Zhang Jie, Wei Yunlin, Chen Chaoyin, Peng Qian

机构信息

Biotechnology Research Center, Faculty of Biological and Chemical Engineering, Kunming University of Science and Technology, Kunming, P.R. China.

出版信息

Can J Microbiol. 2005 Oct;51(10):881-6. doi: 10.1139/w05-087.

Abstract

Several Thermus strains were isolated from 10 hot springs of the Rehai geothermal area in Tengchong, Yunnan province. The diversity of Thermus strains was examined by sequencing the 16S rRNA genes and comparing their sequences. Phylogenetic analysis showed that the 16S rDNA sequences from the Rehai geothermal isolates form four branches in the phylogenetic tree and had greater than 95.9% similarity in the phylogroup. Secondary structure comparison also indicated that the 16S rRNA from the Rehai geothermal isolates have unique secondary structure characteristics in helix 6, helix 9, and helix 10 (reference to Escherichia coli). This research is the first attempt to reveal the diversity of Thermus strains that are distributed in the Rehai geothermal area.

摘要

从云南省腾冲热海地热区的10个温泉中分离出了几株嗜热栖热菌菌株。通过对16S rRNA基因进行测序并比较其序列,研究了嗜热栖热菌菌株的多样性。系统发育分析表明,来自热海地热区分离株的16S rDNA序列在系统发育树中形成四个分支,且在系统发育群中的相似度大于95.9%。二级结构比较还表明,来自热海地热区分离株的16S rRNA在螺旋6、螺旋9和螺旋10(参照大肠杆菌)中具有独特的二级结构特征。本研究首次尝试揭示分布于热海地热区的嗜热栖热菌菌株的多样性。

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