通过与超免疫兔血清进行免疫印迹分析，对不同分离株的特定阴道毛滴虫靶抗原进行表征。

Characterization of specific Trichomonas vaginalis target antigens from different isolates by immunoblotting against hyperimmune rabbit serum.

作者信息

el-Okbi Laila M, Khalifa Khalifa E, Elwakil Hala S, Mohamed Anhar A, Abdel-Hameed Dina M, Tawfik Rania A

机构信息

Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

出版信息

J Egypt Soc Parasitol. 2005 Dec;35(3):891-8.

PMID:16333897

Abstract

The SDS-PAGE and immunoblot methods were used to identify Trichomonas vaginalis specific target antigen(s). Ten T. vaginalis isolates, cultured on TYM media, were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE), revealed 34 distinct bands. Immunoblotting against a hyperimmune rabbit serum, showed 20 reactive bands ranging from 14-205 kDa. There was isolate to isolate variability among 8 isolates, while 2 isolates (6,7) showed a similar antigenic patterns. Imunoblotting of the isolates showed a total of 20 molecular weight bands, 80% of isolates gave positive immunologic reactions above 100 kDa, while below 100 Kda all the isolates recognized the different molecular weight bands. Out of 20 reacting bands, 5 main bands were detected, 29, 66, 84, 95 & 115 kDa gave positive percent of 60, 60, 60, 90 & 80% among the 10 isolates respectively.

摘要

采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法鉴定阴道毛滴虫特异性靶抗原。在TYM培养基上培养的10株阴道毛滴虫分离株，经十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，显示出34条不同的条带。用超免疫兔血清进行免疫印迹，显示出20条反应条带，分子量范围为14 - 205 kDa。8株分离株之间存在分离株间的变异性，而2株分离株（6、7）表现出相似的抗原模式。对分离株的免疫印迹显示共有20条分子量条带，80%的分离株在100 kDa以上产生阳性免疫反应，而在100 kDa以下，所有分离株识别不同分子量的条带。在20条反应条带中，检测到5条主要条带，29、66、84、95和115 kDa在10株分离株中的阳性百分比分别为60%、60%、60%、90%和80%。