Liu Rong-Tao, Xiao Jing, Guo Hui-Ling, Qiu Dun-Guo, Yin Hua-Hu, Wang Zheng-Rong
Biomedical Engineering Department, West China School of Preclinical and Forensic Medicine, Sichuang University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):776-8.
To investigate the expression of A-type atrial natriuretic peptide receptor (ANPR-A) in the kidneys of renovascular hypertension rats and evaluate the significance of the expression.
The rat model of renovascular hypertension was produced by constricting one lateral renal artery. After the renal artery being constricted for 4 weeks and 8 weeks, the systolic BP of rats was measured with a manometer using the tail-cuff method. Then, the expression of ANPR-A was respectively detected by immunohistochemical technique in the kidneys of the two-kidney, one-clip (2K1C) rats, and the expression level of ANPR-A was semi-quantitatively measured by Mias-2000 computer image analyzer.
At 4 weeks after the artery-constricted operation,the expression of ANPR-A increased significantly in 2K1C hypertensive rat glomeruli and decreased significantly in renal tubules, compared with control (P<0.01), but there was no marked change in medullar collecting tubules. At 8 weeks after the artery-constricted operation, the expression of ANPR-A decreased significantly in 2K1C hypertensive rat renal tubules and medullar collecting tubules, compared with control (P<0.01); however, there was weak expression in glomeruli, and no statistically significant difference was seen when compared with control (P>0.05).
The expression of ANPR-A decreased significantly in kidney tissues of renovascular
探讨A型心房利钠肽受体（ANPR-A）在肾血管性高血压大鼠肾脏中的表达情况，并评估其表达的意义。
通过缩窄一侧肾动脉制备肾血管性高血压大鼠模型。肾动脉缩窄4周和8周后，采用尾套法用血压计测量大鼠收缩压。然后，用免疫组织化学技术分别检测双肾单夹（2K1C）大鼠肾脏中ANPR-A的表达，并用Mias-2000计算机图像分析仪对ANPR-A的表达水平进行半定量测定。
动脉缩窄术后4周，与对照组相比，2K1C高血压大鼠肾小球中ANPR-A的表达显著增加，肾小管中显著降低（P<0.01），但髓质集合管无明显变化。动脉缩窄术后8周，与对照组相比，2K1C高血压大鼠肾小管和髓质集合管中ANPR-A的表达显著降低（P<0.01）；然而，肾小球中表达较弱，与对照组相比无统计学差异（P>0.05）。
肾血管性高血压大鼠肾组织中ANPR-A的表达显著降低。
