Zhang Zhuo, Wang Ting-Hua, Zhu Yu-Hong, Feng Zhong-Tang, Sun Bing, Hu Yan-li
The Second Affiliated Hospital of Kunming Medical College, Kunming 650031, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):789-91, 833.
To investigate the distribution and change of NGF, BDNF and NT3 in hippocampus of rat with Alzheimer disease(AD) by immunohistochemistry.
AD model was established by injecting beta amyloid protein into the hippocampus of rat. The rats were killed ten days after injection. The hippocampus sections were made coronally on a freezing microtome. Brain sections were processed by immunohistochemical procedure with molocloned antibodies against NGF, BDNF and NT3. The number of positive neurons of NGF, BDNF and NT3 was counted and analyzed statistically.
It was found that the number of NGF positive neurons increased and the immunostaining intensity became stronger as compared with the control (P<0.01). The number of BDNF positive neurons decreased and the immunostaining intensity weakened (P<0.01). There Our was no change in the number and immunostaining intensity of NT3 positive neurons (P>0.05).
results show that NGF, BDNF, NT3 have experienced different changes in hippocampus of rat with AD, suggesting that they play different roles in the course of AD and bear relation to the physiological function of cholinergic neurons in hippocampus of rat with AD. In particular, BDNF exerts crucial effect on the degeneration of neuronal function in the hippocampus of rat with AD.
采用免疫组织化学方法研究阿尔茨海默病（AD）大鼠海马中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养素-3（NT3）的分布及变化。
通过向大鼠海马内注射β淀粉样蛋白建立AD模型。注射后10天处死大鼠。用冰冻切片机冠状切取海马组织切片。采用抗NGF、BDNF和NT3的单克隆抗体，通过免疫组织化学方法处理脑切片。对NGF、BDNF和NT3阳性神经元数量进行计数并进行统计学分析。
发现与对照组相比，NGF阳性神经元数量增加，免疫染色强度增强（P<0.01）。BDNF阳性神经元数量减少，免疫染色强度减弱（P<0.01）。NT3阳性神经元数量及免疫染色强度无变化（P>0.05）。
结果表明，在AD大鼠海马中，NGF、BDNF、NT3发生了不同变化，提示它们在AD病程中发挥不同作用，且与AD大鼠海马胆碱能神经元的生理功能有关。特别是，BDNF对AD大鼠海马神经元功能退化起关键作用。
Zotero 插件