Wang Xiao-Qin, Su Xu, Liu Han-Min, Gao Ju, Li Feng-Yi, Dong Li-Qun, Luo Chun-Hua
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):804-7.
To investigate the effect of truncated PDGF-alpha receptor on proliferation expression of c-sis mRNA of pulmonary artery vascular smooth muscular cells(VSMC).
Tissue mass culture was done to get vascular smooth muscular cells of pulmonary artery. Different concentrations of truncated PDGF-alpha receptor and adenoviral recombined body (Ad5CMV-PalphaRtr, ACP) were added into the cultures. VSMC proliferation curves were obtained using MTT test. The expression of c-sis mRNA was detected by PCR.
ACP at 5 ml/L and 10 ml/L concentrations restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. The curve presented ascending tendency only after 7 days. Affected by 5 ml/L ACP, PDGF-BB exerted no significant accelerating effect on the proliferation of VSMC. The expression of c-sis mRNA was up-regulated under the effect of ACP. Affected by 5 ml/L ACP and PDGF-BB, the expression of c-sis mRNA was down-regulated.
ACP at 5 ml/L and 10 ml/L concentration powerful inhibitor of cellular proliferation for pulmonary artery VSMC. It can increase c-sis mRNA expression significantly and the increase seems to be ACP dosage dependent.
研究截短型血小板衍生生长因子α受体（PDGF-α受体）对肺动脉血管平滑肌细胞（VSMC）增殖及c-sis mRNA表达的影响。
采用组织块培养法获取肺动脉血管平滑肌细胞，向培养物中加入不同浓度的截短型PDGF-α受体及腺病毒重组体（Ad5CMV-PalphaRtr，ACP）。采用MTT法绘制VSMC增殖曲线，通过PCR检测c-sis mRNA的表达。
5 ml/L和10 ml/L浓度的ACP明显抑制VSMC的增殖，细胞生长峰值降低或消失，曲线仅在7天后呈上升趋势。在5 ml/L ACP作用下，血小板源性生长因子BB（PDGF-BB）对VSMC的增殖无明显促进作用。ACP作用下c-sis mRNA的表达上调，在5 ml/L ACP和PDGF-BB共同作用下，c-sis mRNA的表达下调。
5 ml/L和10 ml/L浓度的ACP是肺动脉VSMC细胞增殖的强效抑制剂，可显著增加c-sis mRNA的表达，且这种增加似乎与ACP剂量有关。
