Miragliotta Vincenzo, Coli Alessandra, Ricciardi Maria P, Podestà Adriano, Abramo Francesca
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Pisa, Italy.
Am J Vet Res. 2005 Nov;66(11):1931-5. doi: 10.2460/ajvr.2005.66.1931.
To compare the distribution of desmoglein (Dsg) 1 and 2 in skin specimens obtained from dogs and cats to provide information about the possible role of the density of Dsg 1 and 2 in the localization of lesions attributable to pemphigus foliaceus in these 2 species.
Skin biopsy specimens obtained from 4 dogs and 4 cats.
Biopsy specimens were collected from the muzzle, bridge of the nose, ear, dorsum, abdomen, area adjacent to the teats, and footpads of each animal. Immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded skin samples by use of a biotinylated mouse monoclonal anti-Dsg 1 and 2 antibody raised against bovine muzzle. Color development was performed by use of the streptavidin-biotin-peroxidase method with a chromogenic substrate.
Immunohistochemical staining yielded a positive reaction in skin samples obtained from all anatomic sites. The intensity and distribution of staining were related to the number of layers of the stratum spinosum. No differences were detected between samples obtained from dogs and cats.
No differences in intensity of Dsg 1 and 2 antigen were observed in the stratum spinosum between skin samples obtained from dogs and cats. Analysis of this result suggests that factors other than the distribution of Dsg may be responsible for the differences in localization of primary clinical lesions in dogs and cats with pemphigus foliaceus.
比较从犬猫获取的皮肤标本中桥粒芯糖蛋白（Dsg）1和2的分布情况，以提供关于Dsg 1和2密度在这两个物种中落叶型天疱疮所致病变定位中可能作用的信息。
从4只犬和4只猫获取的皮肤活检标本。
从每只动物的口鼻部、鼻梁、耳部、背部、腹部、乳头附近区域和脚垫采集活检标本。对用福尔马林固定、石蜡包埋的皮肤样本，使用针对牛口鼻部产生的生物素化小鼠抗Dsg 1和2单克隆抗体进行免疫组织化学分析。采用链霉亲和素 - 生物素 - 过氧化物酶法和显色底物进行显色。
免疫组织化学染色在从所有解剖部位获取的皮肤样本中产生阳性反应。染色强度和分布与棘层的层数有关。在从犬和猫获取的样本之间未检测到差异。
在从犬猫获取的皮肤样本的棘层中，未观察到Dsg 1和2抗原强度的差异。对该结果的分析表明，除Dsg分布外的其他因素可能是犬猫落叶型天疱疮原发性临床病变定位差异的原因。
