Hee A K W, Tan K H
School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.
Bull Entomol Res. 2005 Dec;95(6):615-20. doi: 10.1079/ber2005392.
Sex pheromonal components of the tephritid fruit fly Bactrocera dorsalis (Hendel), 2-allyl-4,5-dimethoxyphenol and (E)-coniferyl alcohol, are biosynthesized from a highly potent male attractant, methyl eugenol, then sequestered and stored in the rectal gland prior to their release during courtship at dusk. These sex pheromonal components have been detected in the haemolymph and crop organ. Hence, attempts were made to separate and identify the haemolymph fractions which contained the sex pheromonal components. Identification of these bioactive fractions in methyl eugenol-fed male flies using gel filtration column chromatography and biodetection using live male flies showed two fractions as highly attractive to conspecific males. These fractions show a significant increase in protein absorbance in the elution profile of haemolymph from methyl eugenol-fed males compared with that from methyl eugenol-deprived males. The molecular mass of these bioactive fractions as determined by using gel filtration was in the peptide range of 3.3 to 5.5 kDa. Subsequent gas chromatography-mass spectrometry analyses further confirmed the presence of the pheromonal components in the bioactive fractions. The presence of these methyl eugenol-derived sex pheromonal components in specific haemolymph fractions suggests the involvement of a sex pheromone binding complex.
桔小实蝇（Bactrocera dorsalis (Hendel)）的性信息素成分，2-烯丙基-4,5-二甲氧基苯酚和（E）-松柏醇，由一种高效的雄性引诱剂甲基丁香酚生物合成，然后在黄昏求偶期间释放之前，被隔离并储存在直肠腺中。这些性信息素成分已在血淋巴和嗉囊器官中被检测到。因此，人们试图分离和鉴定含有性信息素成分的血淋巴组分。使用凝胶过滤柱色谱法对喂食甲基丁香酚的雄性果蝇的血淋巴进行分离，并通过活体雄性果蝇进行生物检测，结果显示有两个组分对同种雄性具有高度吸引力。与未喂食甲基丁香酚的雄性果蝇的血淋巴洗脱图谱相比，喂食甲基丁香酚的雄性果蝇的血淋巴洗脱图谱中蛋白质吸光度显著增加。通过凝胶过滤测定，这些生物活性组分的分子量在3.3至5.5 kDa的肽范围内。随后的气相色谱-质谱分析进一步证实了生物活性组分中存在性信息素成分。这些源自甲基丁香酚的性信息素成分在特定血淋巴组分中的存在表明存在一种性信息素结合复合物。
