Verdida R A, Xuan X, Fukumoto S, Huang X, Zhou J, Igarashi I, Claveria F G, Nagasawa H
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Parasitology. 2005 Dec;131(Pt 6):769-74. doi: 10.1017/S0031182005008401.
An immunochromatographic test (ICT), using recombinant truncated P50 (P50t), for the detection of antibodies to Babesia gibsoni was developed and evaluated. Whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with B. gibsoni were clearly positive in the ICT. In addition, the ICT detected no cross-reactivity with sera from dogs experimentally infected with closely related parasites, B. canis canis, B. canis vogeli, and B. canis rossi, or with Neospora caninum, and Leishmania infantum. Sequential sera from a dog experimentally infected with B. gibsoni were tested with the ICT; it was shown that the specific antibodies are detectable as early as 6 days post-infection (p.i.) and that strong antibody responses remained until the end of the experiment (144 days p.i.). To evaluate the clinical application of the ICT, a total of 54 serum samples collected from domestic dogs that had been identified as having signs of anaemia at veterinary hospitals in Japan, were tested with the ICT, the previously established enzyme-linked immunosorbent assay (ELISA) and with the indirect fluorescent antibody test (IFAT). Twenty-four of the tested samples (44.4%) were positive in both ICT and ELISA, and (51.8%) in IFAT. The concordance between ELISA and ICT was found to be 100%, and 85.7% with IFAT. Taken together, the results above suggest that the ICT using P50t is rapid, simple, accurate, and suitable for use at clinical sites for the diagnosis of B. gibsoni infection in dogs.
开发并评估了一种使用重组截短型P50（P50t）的免疫层析试验（ICT），用于检测吉氏巴贝斯虫抗体。所有来自无特定病原体犬的血清在ICT中均呈明显阴性，而所有来自经吉氏巴贝斯虫实验感染犬的血清在ICT中均呈明显阳性。此外，ICT未检测到与经密切相关寄生虫犬巴贝斯虫犬亚种、犬巴贝斯虫韦氏亚种和犬巴贝斯虫罗氏亚种实验感染犬的血清，或与犬新孢子虫和婴儿利什曼原虫血清的交叉反应。用ICT检测了一只经吉氏巴贝斯虫实验感染犬的系列血清；结果显示，特异性抗体最早在感染后6天即可检测到，并且在实验结束时（感染后144天）仍保持强烈的抗体反应。为了评估ICT的临床应用，对从日本兽医医院收集的54份已确定有贫血症状的家犬血清样本进行了ICT检测、先前建立的酶联免疫吸附试验（ELISA）和间接荧光抗体试验（IFAT）。24份检测样本（44.4%）在ICT和ELISA中均呈阳性，在IFAT中呈阳性的比例为（51.8%）。发现ELISA与ICT之间的一致性为100%，与IFAT的一致性为85.7%。综上所述，上述结果表明，使用P50t的ICT快速、简单、准确，适用于临床现场诊断犬的吉氏巴贝斯虫感染。
