de Jong Koert P, Vermeulen Peter B, van Marck Eric, Boot Marcel, Gouw Annette S H
Department of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University Medical Center Groningen, University of Groningen, PO Box 30.001, 9700 RB Groningen, The Netherlands.
Eur J Cancer. 2006 Jan;42(1):97-100. doi: 10.1016/j.ejca.2005.09.019. Epub 2005 Dec 5.
Standardised methods of microvessel density quantification have been published. However, a reliable and reproducible method to visualise endothelial cell apoptosis is lacking, which is a shortcoming in assessing vascular remodelling during angiogenesis. The aim of this study was to validate a newly developed technique to demonstrate endothelial cell apoptosis by double immunolabelling with anti-CD34 and anti-activated caspase-3 in human adenocarcinomas. Double immunolabelling was used to identify apoptotic endothelial cells in six tumours of eight different human adenocarcinomas. Microvessel density and rate of apoptotic endothelial cells were quantified. The technique revealed endothelial cell apoptosis simultaneously with the identification of microvessel density on one slide. These characteristics were reproducible in adenocarcinomas of various sites. In conclusion, apoptotic endothelial cells and microvessel density can now be evaluated simultaneously within one and the same area, allowing a more reliable histological quantification of angiogenesis.
微血管密度定量的标准化方法已经公布。然而，目前缺乏一种可靠且可重复的方法来可视化内皮细胞凋亡，这是评估血管生成过程中血管重塑的一个不足之处。本研究的目的是验证一种新开发的技术，该技术通过用抗CD34和抗活化半胱天冬酶-3进行双重免疫标记来证明人腺癌中的内皮细胞凋亡。使用双重免疫标记来鉴定来自8种不同人腺癌的6个肿瘤中的凋亡内皮细胞。对微血管密度和凋亡内皮细胞率进行了定量。该技术在一张载玻片上同时显示了内皮细胞凋亡并鉴定了微血管密度。这些特征在不同部位的腺癌中具有可重复性。总之，现在可以在同一区域内同时评估凋亡内皮细胞和微血管密度，从而对血管生成进行更可靠的组织学定量。
