Marenzana Massimo, Wilson-Jones Nick, Mudera Vivek, Brown Robert A
University College London, RFUCMS, Tissue Repair and Engineering Centre, Institute of Orthopaedics, RNOH, Stanmore Campus, London HA7 4LP, UK.
Exp Cell Res. 2006 Feb 15;312(4):423-33. doi: 10.1016/j.yexcr.2005.11.005. Epub 2005 Dec 6.
The absence of a controllable in vitro model of soft tissue remodeling is a major impediment, limiting our understanding of collagen pathologies, tissue repair and engineering. Using 3D fibroblast-collagen lattice model, we have quantified changes in matrix tension and material properties following remodeling by blockade of cell-generated tension with cytochalasin D. This demonstrated a time-dependent shortening of the collagen network, progressively stabilized into a built-in tension within the matrix. This was differentially enhanced by TGFB1 and mechanical loading to give subtle control of the new, remodeled matrix material properties. Through this model, we have been able to identify the 'tension remodeling' process, by which cells control material properties in response to environmental factors.
缺乏可控的软组织重塑体外模型是一个主要障碍，限制了我们对胶原蛋白病理学、组织修复和工程的理解。使用三维成纤维细胞-胶原晶格模型，我们通过用细胞松弛素D阻断细胞产生的张力，量化了重塑后基质张力和材料特性的变化。这表明胶原网络呈时间依赖性缩短，逐渐稳定为基质内的固有张力。转化生长因子β1（TGFB1）和机械加载对此有不同程度的增强作用，从而对新的重塑基质材料特性进行精细控制。通过这个模型，我们已经能够识别“张力重塑”过程，即细胞根据环境因素控制材料特性的过程。
