Wherrett Tim, Shabala Sergey, Pottosin Igor
School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tas 7001, Australia.
FEBS Lett. 2005 Dec 19;579(30):6890-4. doi: 10.1016/j.febslet.2005.11.038. Epub 2005 Dec 1.
Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 microM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.
膜片钳实验表明，近等基因的ET8（耐铝）和ES8（铝敏感）小麦品种在慢液泡通道特性上存在显著差异。在对照条件下，ES8中全液泡电流的失活更快。施加1.4微摩尔的液泡Al3+会使ET8的激活阈值升高20毫伏，并减缓其激活动力学，但ES8中则不会。ES8中的通道密度比ET8高约30%，并且不受24小时铝预处理的影响。然而，铝预处理的ES8激活阈值降低。总体而言，我们的数据表明，Alt1位点可能控制的不仅仅是小麦中的质膜苹果酸通道。
