Ma Ming, Luo Xubiao, Chen Bo, Su Shengpei, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
J Chromatogr A. 2006 Jan 20;1103(1):170-6. doi: 10.1016/j.chroma.2005.11.061. Epub 2005 Dec 7.
An accurate method was developed for the simultaneous determination of water-Tartrazine, Amaranth, Ponceau 4R, Sunset Yellow FCF, and fat-Sudan (I-IV), synthetic soluble colorants in foodstuff. This method uses dimethylsulfoxide (DMSO) as the extraction solvent in the sample preparation process and high performance liquid chromatography (HPLC)-diode array detector (DAD)-electrospray mass spectrometry (ESI-MS), applying selected ion recording in positive/negative alternate mode to acquire mass spectral data, as the analytical technique. Linearity of around three orders in the magnitude of concentration was generally obtained. Detection and quantification limits of the investigated dyes, which were evaluated at signal to noise ratio of 3 for detection limit and 10 for quantification limit, were in the ranges of 0.01-4 and 0.03-11.2 ng, respectively. The recoveries of the eight synthetic colorants in four matrices ranged from 93.2 to 108.3%. Relative standard deviations of less than 8.2% were also achieved. This method has been applied successfully in the determination of water-soluble colorants in the soft drink and the delicious ginger, and fat-soluble dyes in chilli powders and chilli spices.
开发了一种准确的方法，用于同时测定食品中的水溶性柠檬黄、苋菜红、胭脂红4R、日落黄FCF和脂溶性苏丹红（I-IV）等合成食用色素。该方法在样品制备过程中使用二甲基亚砜（DMSO）作为萃取溶剂，并采用高效液相色谱（HPLC）-二极管阵列检测器（DAD）-电喷雾质谱（ESI-MS），以正/负交替模式应用选择离子记录来获取质谱数据作为分析技术。浓度范围一般在三个数量级左右呈现线性关系。所研究染料的检测限和定量限分别以信噪比为3计算检测限、信噪比为10计算定量限，范围分别为0.01 - 4 ng和0.03 - 11.2 ng。四种基质中八种合成色素的回收率在93.2%至108.3%之间。相对标准偏差也小于8.2%。该方法已成功应用于软饮料和美味姜中水溶性色素以及辣椒粉和辣椒香料中脂溶性色素的测定。
