绘制子囊菌酵母齐藤氏土星孢中3-羟基氧化脂质的分布图谱。
Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi.
作者信息
Bareetseng Andries S, Kock Johan L F, Pohl Carolina H, Pretorius Elma E, Strauss Catharina J, Botes Piet J, Van Wyk Pieter W J, Nigam Santosh
机构信息
Department of Microbial, Biochemical and Food Biotechnology, UNESCO MIRCEN: Industrial Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, South Africa.
出版信息
Syst Appl Microbiol. 2006 Sep;29(6):446-9. doi: 10.1016/j.syapm.2005.11.008. Epub 2005 Dec 7.
The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores.
利用免疫荧光显微镜对斋藤土星孢中3-羟基氧化脂质的分布进行了定位。在聚集的子囊孢子上观察到荧光，表明子囊孢子表面或之间存在3-羟基氧化脂质。使用气相色谱-质谱法将氧化脂质鉴定为3-羟基-9:1。此外，利用扫描电子显微镜和透射电子显微镜对子囊孢子进行的超微结构研究显示，椭圆形子囊孢子周围有明显的赤道壁架。
Zotero 插件