胃癌组织中蛋白酪氨酸磷酸酶的表达谱分析

Protein tyrosine-phosphatase expression profiling in gastric cancer tissues.

作者信息

Wu Chew-Wun, Kao Hwa-Li, Li Anna F-Y, Chi Chin-Wen, Lin Wen-Chang

机构信息

Department of Surgery, Veterans General Hospital, Taipei, Taiwan, ROC.

出版信息

Cancer Lett. 2006 Oct 8;242(1):95-103. doi: 10.1016/j.canlet.2005.10.046. Epub 2005 Dec 7.

DOI:10.1016/j.canlet.2005.10.046

PMID:16338072

Abstract

Protein phosphorylation is an important regulatory mechanism involved in signal transduction and cancers. In comparison to the extensive tyrosine-kinase oncogenesis research, there are only relatively few studies of protein tyrosine-phosphatase expression in cancers. The expression profile for tyrosine-phosphatases was investigated in gastric cancers using RT-PCR and molecular cloning. The present study showed a general PTP expression profile in gastric cancer tissues, with the identification of 22 distinct tyrosine-phosphatases. Following the examination of five PTPs (PTPRA, PTPRB, PTPRD, PTPRG and PTPRZ) using immunohistochemistry, strong association was observed between PTPRA/PTPRZ expression and gastric cancer progression including lymphovascular invasion and liver/peritoneal dissemination.

摘要

蛋白质磷酸化是一种参与信号转导和癌症的重要调节机制。与广泛的酪氨酸激酶致癌作用研究相比，关于蛋白质酪氨酸磷酸酶在癌症中表达的研究相对较少。使用逆转录聚合酶链反应（RT-PCR）和分子克隆技术，对胃癌中酪氨酸磷酸酶的表达谱进行了研究。本研究显示了胃癌组织中酪氨酸磷酸酶的总体表达谱，共鉴定出22种不同的酪氨酸磷酸酶。在使用免疫组织化学检查了5种酪氨酸磷酸酶（PTPRA、PTPRB、PTPRD、PTPRG和PTPRZ）之后，观察到PTPRA/PTPRZ表达与胃癌进展（包括淋巴管侵犯和肝/腹膜转移）之间存在密切关联。