Boorer K J, Forde B G, Leigh R A, Miller A J
Department of Biochemistry and Physiology, AFRC-IACR, Harpenden, Herts., UK.
FEBS Lett. 1992 May 11;302(2):166-8. doi: 10.1016/0014-5793(92)80431-f.
A full-length cDNA clone for the H+/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STP1 mRNA accumulated 3-O-[methyl-14C]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H+/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.
