Mellado V, Yáñez M A, Catalán V
Labaqua, Pol. Ind. Las Atalayas, C/Del Dracma 16-18, 03114 Alicante, Spain.
Microbiol Res. 2006;161(1):20-4. doi: 10.1016/j.micres.2005.04.001. Epub 2005 Jun 1.
In this study, the performance of the MicroFoss system (Foss, Spain) for the enumeration of Escherichia coli in water samples was evaluated. One hundred and eighty-five samples were analysed both by MicroFoss assay and culture isolation on Tryptone-Bile X-glucuronide agar (TBX), and the correlation coefficient obtained was 0.92. The analysis of 28 new samples using both methods showed a statistically significant relationship at the 99.5% confidence level between log colony forming units obtained by MicroFoss assay and those obtained using growth on TBX agar. Nevertheless, when the level of sample contamination was low, the variability was high. In conclusion, the MicroFoss system is a rapid and simple alternative method for the enumeration of E. coli in water although discordance between the results using these methods in samples with low counts could limit its use for the study of clean water such as potable water.
在本研究中，对MicroFoss系统（西班牙福斯公司）用于水样中大肠杆菌计数的性能进行了评估。采用MicroFoss分析法和在胰蛋白胨-胆汁X-葡糖醛酸琼脂（TBX）上进行培养分离的方法对185个样品进行了分析，得到的相关系数为0.92。使用这两种方法对28个新样品进行分析，结果表明，在99.5%置信水平下，通过MicroFoss分析法获得的对数菌落形成单位与在TBX琼脂上生长获得的对数菌落形成单位之间存在统计学显著关系。然而，当样品污染水平较低时，变异性较高。总之，MicroFoss系统是一种快速简便的替代方法，可用于水样中大肠杆菌的计数，尽管在低计数样品中使用这些方法得到的结果不一致，可能会限制其在诸如饮用水等清洁水研究中的应用。
