Slakeski N, Fincher G B
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
FEBS Lett. 1992 Jul 20;306(2-3):98-102. doi: 10.1016/0014-5793(92)80977-o.
Treatment of young barley leaves with indole acetic acid (IAA) or gibberellic acid (GA3) results in a dramatic increase in levels of (1----3,1----4)-beta-glucanase isoenzyme EI transcripts. In young roots of comparable age, levels of isoenzyme EI mRNA are high; IAA inhibits expression while GA3 has no effect on mRNA levels. The addition of both abscisic acid and GA3 to leaves, roots and aleurone layers leads to higher levels of (1----3,1----4)-beta-glucanase isoenzyme EI mRNA than is found with Ga3 alone. Little or no expression of (1----3,1----4)-beta-glucanase isoenzyme EII is detected in vegetative tissues, but in isolated aleurone layers GA3 enhances levels of isoenzyme EII transcripts, as does IAA. Thus, the two barley (1----3,1----4)-beta-glucanase genes respond quite differently to phytohormone treatment, depending on the tissue and its stage of development.
用吲哚乙酸（IAA）或赤霉素（GA3）处理大麦幼叶会导致（1→3，1→4）-β-葡聚糖酶同工酶EI转录本水平急剧增加。在年龄相当的幼根中，同工酶EI mRNA水平较高；IAA抑制其表达，而GA3对mRNA水平没有影响。将脱落酸和GA3同时添加到叶片、根和糊粉层中，会使（1→3，1→4）-β-葡聚糖酶同工酶EI mRNA水平高于单独使用GA3时的水平。在营养组织中几乎检测不到（1→3，1→4）-β-葡聚糖酶同工酶EII的表达，但在分离的糊粉层中，GA3会提高同工酶EII转录本的水平，IAA也有同样的作用。因此，大麦的两个（1→3，1→4）-β-葡聚糖酶基因对植物激素处理的反应差异很大，这取决于组织及其发育阶段。
