Onaka Hiroyasu, Nakaho Mizuho, Hayashi Keiko, Igarashi Yasuhiro, Furumai Tamotsu
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
Microbiology (Reading). 2005 Dec;151(Pt 12):3923-3933. doi: 10.1099/mic.0.28420-0.
The biosynthetic gene cluster of goadsporin, a polypeptide antibiotic containing thiazole and oxazole rings, was cloned from Streptomyces sp. TP-A0584. The cluster contains a structural gene, godA, and nine god (goadsporin) genes involved in post-translational modification, immunity and transcriptional regulation. Although the gene organization is similar to typical bacteriocin biosynthetic gene clusters, each goadsporin biosynthetic gene shows low homology to these genes. Goadsporin biosynthesis is initiated by the translation of godA, and the subsequent cyclization, dehydration and acetylation are probably catalysed by godD, godE, godF, godG and godH gene products. godI shows high similarity to the 54 kDa subunit of the signal recognition particle and plays an important role in goadsporin immunity. Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of godA, suggesting that this biosynthesis machinery is used for the heterocyclization of peptides.
从链霉菌属TP - A0584中克隆得到了含有噻唑环和恶唑环的多肽抗生素刺孢霉素的生物合成基因簇。该基因簇包含一个结构基因godA以及九个参与翻译后修饰、免疫和转录调控的god（刺孢霉素）基因。尽管该基因组织与典型的细菌素生物合成基因簇相似，但每个刺孢霉素生物合成基因与这些基因的同源性较低。刺孢霉素的生物合成由godA的翻译起始，随后的环化、脱水和乙酰化可能由godD、godE、godF、godG和godH基因产物催化。godI与信号识别颗粒的54 kDa亚基高度相似，并在刺孢霉素免疫中发挥重要作用。此外，通过对godA进行定点诱变产生了四种刺孢霉素类似物，表明这种生物合成机制用于肽的杂环化。
