通过移植到无胸腺裸鼠体内的人分泌生长激素垂体腺瘤进行体内125I-生长激素释放激素（GHRH）结合的放射自显影证明。

Autoradiographic demonstration of in vivo 125I-growth hormone-releasing hormone (GHRH) binding by human GH-secreting pituitary adenomas transplanted on athymic nude mice.

作者信息

Puchner M J, Lüdecke D K, Knappe U J, Saeger W, Herrmann H D

机构信息

Department of Neurosurgery, University of Hamburg, Germany.

出版信息

Mol Cell Endocrinol. 1992 Jun;85(3):157-64. doi: 10.1016/0303-7207(92)90254-4.

DOI:10.1016/0303-7207(92)90254-4

PMID:1634014

Abstract

This study was designed for the purpose of investigating a method for in vivo tumor labelling of human GH (hGH) secreting pituitary adenomas. Pituitary adenoma tissue removed from four acromegalic patients was transplanted into 62 athymic nude mice. After positive GHRH stimulation tests 125I-GHRH(1-44) NH2 was injected intravenously (i.v.) in ten nude mice. 10 min after 125I-GHRH injection, the nude mice were sacrificed, the transplants excised and prepared for light microscopical autoradiography. The mouse pituitary and skeletal muscle specimens served as controls. After the i.v. injection of 125I-GHRH we observed a marked accumulation of silver grains within the adenoma tissue indicating tumor labelling. This study is a first step in investigating a new method for labelling small residues of hGH secreting pituitary adenomas intraoperatively.

摘要

本研究旨在探索一种对分泌人生长激素（hGH）的垂体腺瘤进行体内肿瘤标记的方法。从4例肢端肥大症患者身上切除的垂体腺瘤组织被移植到62只无胸腺裸鼠体内。在进行阳性生长激素释放激素（GHRH）刺激试验后，给10只裸鼠静脉注射125I-GHRH(1-44) NH2。注射125I-GHRH 10分钟后，处死裸鼠，切除移植组织并制备用于光学显微镜放射自显影。小鼠垂体和骨骼肌标本作为对照。静脉注射125I-GHRH后，我们观察到腺瘤组织内有明显的银粒积累，表明肿瘤被标记。本研究是探索术中标记分泌hGH的垂体腺瘤小残留组织新方法的第一步。