微米级未染色动脉壁结构的体外多光子成像
Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure.
作者信息
Boulesteix T, Pena A-M, Pagès N, Godeau G, Sauviat M-P, Beaurepaire E, Schanne-Klein M-C
机构信息
Laboratory for Optics and Biosciences, CNRS/INSERM, Ecole Polytechnique, 91128 Palaiseau, France.
出版信息
Cytometry A. 2006 Jan;69(1):20-6. doi: 10.1002/cyto.a.20196.
BACKGROUND
We characterize the application of multiphoton microscopy to the observation of the extracellular matrix of fresh unstained vessels.
METHOD
Combined two-photon-excited fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable specificity.
RESULTS
We present elastin/collagen imaging in unstained rat vessels at both micrometer and whole vessel scales, and we characterize the optical properties of rat carotid artery and aorta walls. We apply this method to evidence deleterious effects of residual doses of a pesticide on the vessel wall.
CONCLUSION
This study illustrates the potential of 2PEF/SHG microscopy for pharmacological studies in unlabeled arteries.
背景
我们描述了多光子显微镜在观察未染色新鲜血管细胞外基质中的应用。
方法
对大动脉进行双光子激发荧光（2PEF）和二次谐波产生（SHG）联合成像，以显著的特异性揭示血管壁中弹性蛋白和胶原纤维的结构。
结果
我们展示了未染色大鼠血管在微米和全血管尺度下的弹性蛋白/胶原成像，并表征了大鼠颈动脉和主动脉壁的光学特性。我们应用该方法证明了农药残留剂量对血管壁的有害影响。
结论
本研究说明了2PEF/SHG显微镜在未标记动脉药理学研究中的潜力。
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