[湖北贝母的高效液相色谱指纹图谱]
[HPLC fingerprint of Fritillaria hupehensis].
作者信息
Zhang Guo-Xin, Li Juan, Zhang Peng, Ruan Han-Li, Zhang Yong-Hui, Pi Hui-Fang, Wu Ji-Zhou
机构信息
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Yao Xue Xue Bao. 2005 Sep;40(9):850-3.
AIM
To establish a fingerprint analysis method of Fritillaria hupehensis.
METHODS
fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1).
RESULTS
HPLC fingerprint of Fritillaria hupehensis was obtained.
CONCLUSION
A reliable method was provided for controlling the quality of Fritillaria hupehensis.
目的
建立湖北贝母的指纹图谱分析方法。
方法
采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法进行指纹图谱研究。使用Hypersil ODS柱；流动相由甲醇（含0.05%三乙胺）和水组成，梯度洗脱；流速为1.0 mL·min⁻¹；记录时间为60 min；漂移管温度为75℃；气体流速为1.9 L·min⁻¹。
结果
获得了湖北贝母的HPLC指纹图谱。
结论
为控制湖北贝母的质量提供了一种可靠的方法。
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