Yu Hua, Jiang Yan, Li Ping, Li Shao-Ping, Wang Yi-Tao
Key Laboratory of Modern Traditional Chinese Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Zhongguo Zhong Yao Za Zhi. 2005 Apr;30(8):572-5.
To develop a HPLC-ELSD method for the quantitation of peimisine in Bulbus Fritillariae Cirrhosae.
HPLC equipped with ELSD was used for the analysis of peimisine in Bulbus Fritillariae Cirrhosae. The separation of peimisine was achieved on a reversed-phase Agilent Hypersil BDS-C18 column with an isocratic phase system consisting of acetonitrile-water-diethylamine (37:63:0. 03) under a flow rate at 1.0 mL x min(-1). Temperature for the detector drift tube was 115 degrees C. The air gas flow rate was 3.0 L x min(-1).
The analysis of peimissine showed good linearity between 0.031 88 - 1.020 mg x mL(-1). The average recovery was 102.1% (n = 5) with RSD 3.86%. According to the results of the 33 different Bulbus Fritillariae Cirrhosae obtained from various origins, the content limit of peimisine was determined to be 0.0070%.
The assay was successfully utilized to quantify the major biologically active alkaloid in four species of Bulbus Fritillariae Cirrhosae. The developed method is simple, rapid, accurate and sensitive for determining the content of peimisine in crude Bulbus Fritillariae Cirrhosae.
建立一种高效液相色谱-蒸发光散射检测法（HPLC-ELSD）测定川贝母中浙贝母碱的含量。
采用配有蒸发光散射检测器的高效液相色谱法分析川贝母中的浙贝母碱。浙贝母碱的分离在反相安捷伦Hypersil BDS-C18柱上进行，等度洗脱体系为乙腈-水-二乙胺（37:63:0.03），流速为1.0 mL·min⁻¹。检测器漂移管温度为115℃，空气流速为3.0 L·min⁻¹。
浙贝母碱在0.031 88 - 1.020 mg·mL⁻¹范围内线性关系良好。平均回收率为102.1%（n = 5），相对标准偏差（RSD）为3.86%。根据从不同产地获得的33份不同川贝母的检测结果，确定浙贝母碱的含量限度为0.0070%。
该方法成功用于定量四种川贝母中主要的生物活性生物碱。所建立的方法用于测定川贝母药材中浙贝母碱的含量，简便、快速、准确、灵敏。
