Wolf Ronni, Grimaldi Elena, Donnarumma Giovanna, Greco Rita, Auricchio Lucia, De Filippis Anna, Tufano Maria Antonietta
Dermitology Unit, Kaplan Medical Center, Rechovot, Isreal.
J Travel Med. 2005 Nov-Dec;12(6):343-6. doi: 10.2310/7060.2005.12608.
The incidence of typhoid fever and shigellosis parallels that of malaria, so many individuals who are on antimalarial drugs can be found in areas where these diseases are widespread. We investigated the effect of quinine sulfate on the growth and invasion of Salmonella typhimurium and Shigella flexneri M90T to determine whether people on antimalarials can have secondary gain from some protection against typhoid fever and shigellosis.
The effect of 50 and 100 microM quinine sulfate on the invasive ability of Salmonella typhimurium and Shigella flexneri M90T into human colon adenocarcinoma-2 (Caco-2) cells was studied during the infection period. The invasive efficiency was expressed as the number of viable internalized bacteria by counting the colony-forming units.
The invasive ability of Salmonella typhimurium and Shigella flexneri M90T was significantly inhibited by 50 and 100 microM quinine sulfate in a dose-dependent manner (for Salmonella typhimurium) when the drug was added to Caco-2 cell monolayers during the infection period.
Since so many people who are on antimalarial drugs visit and inhabit areas that are endemic to typhoid fever and Shigella infection, a study on the influence of these drugs on the disease is long overdue. Our data indicate that quinine sulfate interferes with the invasion and internalization of Salmonella typhimurium and Shigella flexneri M90T into host cells. Further studies on additional strains/serotypes with other newer antimalarials at various concentrations are needed to verify this effect of quinine sulfate and to draw conclusions on its significance in vivo.
伤寒热和志贺氏菌病的发病率与疟疾相似，因此在这些疾病广泛流行的地区可以发现许多正在服用抗疟药物的人。我们研究了硫酸奎宁对鼠伤寒沙门氏菌和宋内志贺氏菌M90T生长和侵袭的影响，以确定服用抗疟药的人是否能从预防伤寒热和志贺氏菌病中获得额外益处。
在感染期间，研究了50和100微摩尔硫酸奎宁对鼠伤寒沙门氏菌和宋内志贺氏菌M90T侵入人结肠腺癌-2（Caco-2）细胞能力的影响。通过计数菌落形成单位，将侵袭效率表示为内化活菌的数量。
当在感染期间将药物添加到Caco-2细胞单层中时，50和100微摩尔硫酸奎宁以剂量依赖的方式（对鼠伤寒沙门氏菌而言）显著抑制了鼠伤寒沙门氏菌和宋内志贺氏菌M90T的侵袭能力。
由于如此多服用抗疟药的人前往并居住在伤寒热和志贺氏菌感染的流行地区，早就应该对这些药物对疾病的影响进行研究。我们的数据表明，硫酸奎宁会干扰鼠伤寒沙门氏菌和宋内志贺氏菌M90T对宿主细胞的侵袭和内化。需要对其他新型抗疟药在不同浓度下对其他菌株/血清型进行进一步研究，以验证硫酸奎宁的这种作用，并就其在体内的意义得出结论。
