Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Kyushu University, Fukuoka 812, Japan.
Appl Environ Microbiol. 1982 May;43(5):1182-7. doi: 10.1128/aem.43.5.1182-1187.1982.
Plate (or slope) cultures of endomycin-producing Streptomyces endus (KCC S-0213) showed spontaneously developing pocks which increased in number during subculturing. Neither spore formation nor typical aerial hyphae formation was observed in the pocks, whereas formation of substrate hyphase was not inhibited. Almost all of the hyphae were broken or lysed in the pocks, and many phage tail tiplike particles were observed in the pocks. No self-replication activity was associated with the particles. The particles often formed a hexagonal crystal or a large crystal mass. The production of these particles did not occur in the liquid culture or in young or normal plate cultures having no pocks. These results were similar to those obtained from the plaque-making phenomenon, except for active phage production, in thiostrepton-producing Streptomyces azureus (ATCC 14921), which has been described previously.
恩多霉素产生菌绛红小单孢菌（KCC S-0213）的平板（或斜面）培养物会自发出现小凹坑，且在传代培养过程中小凹坑数量会增加。在小凹坑中既观察不到孢子形成，也观察不到典型的气生菌丝形成，而基质菌丝的形成并未受到抑制。小凹坑中的大多数菌丝都被破坏或裂解，并且观察到许多噬菌体尾状尖端样颗粒。这些颗粒与自我复制活性无关。这些颗粒通常形成六方晶体或大的晶体团块。这些颗粒的产生不会发生在液体培养物中，也不会发生在没有小凹坑的年轻或正常平板培养物中。这些结果与先前描述的硫链丝菌素产生菌绛红小单孢菌（ATCC 14921）的噬菌斑形成现象相似，只是没有活跃的噬菌体产生。
