利用 Hoechst 33258 荧光光度法测定水中微生物的 DNA。
Fluorometric determination of DNA in aquatic microorganisms by use of hoechst 33258.
机构信息
Environmental Biology Branch, Naval Research Laboratory, Washington, D.C. 20375.
出版信息
Appl Environ Microbiol. 1982 Jun;43(6):1393-9. doi: 10.1128/aem.43.6.1393-1399.1982.
Abstract
A method for the determination of microbial DNA in aquatic environments by the use of Hoechst 33258 has been developed. With unsophisticated instrumentation and simple extraction procedures, it is possible to detect from 0.05 to 10 mug of DNA in bacterial cultures or natural water samples. The method is specific for DNA; DNase I treatment of extracts of natural microbial populations removed 95 to 100% of the observed fluorescence. DNA content ranged from 165 ng ml for relatively eutrophic Potomac River water to 27 ng ml for coastal Atlantic Ocean water and was correlated to an acridine orange direct count (r = 0.90).
摘要
已经开发出一种通过使用 Hoechst 33258 来测定水生态环境中微生物 DNA 的方法。通过使用简单的仪器和简单的提取程序，有可能从细菌培养物或天然水样中检测到 0.05 至 10 微克的 DNA。该方法对 DNA 具有特异性；天然微生物群体提取物的 DNase I 处理去除了 95%至 100%的观察到的荧光。DNA 含量范围从相对富营养化的波托马克河水中的 165ng/ml 到沿海水域的大西洋水中的 27ng/ml，并且与吖啶橙直接计数相关（r=0.90）。
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