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基于 DNA 杂交的淡水细菌浮游生物的站点间比较。

Between-site comparison of freshwater bacterioplankton by DNA hybridization.

机构信息

Department of Applied Biology, University of Hull, HU6 7RX, Hull, England.

出版信息

Microb Ecol. 1993 Nov;26(3):189-200. doi: 10.1007/BF00176952.

DOI:10.1007/BF00176952
PMID:24190089
Abstract

Natural assemblages of freshwater bacterioplankton in enriched lowland rivers had greater cell-specific metabolic activity than those in gravel-pit ponds. Similarly, cell-specific activity and mean cell Size in calcareous headstreams tended to be greater than in intermittently-acid headstreams on millstone grit. DNA was extracted and purified from bacterioplankton assemblages, and between-site comparisons were made in terms of percentage similarity as indicated by DNA hybridization. Cluster analysis, using percentage-similarity matrices, placed bacterioplankton assemblages from different site types into distinct groups. This suggested that between-site physiological differences were related to intrinsically different bacterial composition rather than to different physiological response to different environmental conditions by essentially similar bacterial assemblages.

摘要

富营养化低地河流中的淡水细菌浮游生物自然组合的细胞特异性代谢活性大于砾石坑池塘中的组合。同样,在片麻岩砾石上,石灰质源头溪流的细胞特异性活性和平均细胞大小往往大于间歇性酸化源头溪流。从细菌浮游生物组合中提取并纯化 DNA,并根据 DNA 杂交所示的百分比相似性进行站点间比较。使用百分比相似性矩阵的聚类分析将不同类型站点的细菌浮游生物组合分为不同的组。这表明站点间的生理差异与固有不同的细菌组成有关,而不是与基本相似的细菌组合对不同环境条件的不同生理反应有关。

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本文引用的文献

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Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies.从天然浮游微生物中提取适合分子生物学研究的 DNA。
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