Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya, 464 Japan, and National Biological Institute, Bogor, Indonesia.
Appl Environ Microbiol. 1984 Jan;47(1):44-8. doi: 10.1128/aem.47.1.44-48.1984.
Detached warty lenticellate bark of a mangrove tree species, Bruguiera gymnorrhiza (L.) Lamk. from Iriomote Island, Okinawa, a subtropical region of Japan, showed development of acetylene reduction activity when incubated in a mineral nutrient solution lacking nitrogen under an atmosphere consisting of 5% O(2), 90% N(2), and 5% C(2)H(2). The bacteria responsible for nitrogen fixation were isolated from the bark, and their capacity for acetylene reduction and the incorporation of N(2) into the bacterial cells was confirmed. Four representative strains of the isolates were subjected to taxonomic classification. Two strains were similar to Enterobacter cloacae, and another resembled Enterobacter aerogenes. The characteristics of the fourth strain were similar to those of Klebsiella planticola (Bagley et al., Curr. Microbiol. 6:105-109, 1981). The results of this investigation suggest that the acetylene reduction activity of lenticellate warts of mangrove trunk bark is due to the presence in the warts of nitrogen-fixing bacteria belonging to the family Enterobacteriaceae.
来自日本冲绳亚热带地区伊里岛的红树物种木榄（Bruguiera gymnorrhiza（L.）Lamk.）的离生疣状具皮孔的树皮，在缺乏氮的矿物质营养溶液中，在由 5%O(2)、90%N(2)和 5%C(2)H(2)组成的大气条件下孵育时，显示出乙炔还原活性的发展。负责固氮的细菌从树皮中分离出来，并证实了它们的乙炔还原能力和将 N(2)掺入细菌细胞的能力。对分离物的四个代表性菌株进行了分类学分类。两种菌株类似于阴沟肠杆菌，另一种类似于产气肠杆菌。第四种菌株的特征与植生克雷伯氏菌（Bagley 等人，Curr. Microbiol. 6:105-109, 1981）相似。该研究的结果表明，红树林树干树皮皮孔的乙炔还原活性是由于皮孔中存在属于肠杆菌科的固氮细菌。
