Department of Biology, University of Houston, Houston, Texas 77004.
Appl Environ Microbiol. 1984 May;47(5):1185-7. doi: 10.1128/aem.47.5.1185-1187.1984.
Quantitative N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) oxidase and superoxide dismutase (SOD) analyses were performed on representative organisms of the family Azotobacteraceae. Azotobacter vinelandii, Azotobacter chroococcum, Azotobacter paspali, and Derxia gummosa exhibited high quantitative TMPD oxidase activities, and their extracts possessed very active and electrophoretically homogeneous (single gel band) Fe-type SODs. Azomonas macrocytogenes extracts had similar single Fe-type SODs, and their cells exhibited no TMPD-dependent cytochrome oxidase activity. Nitrogen-fixing cells of Beijerinckia indica, Beijerinckia derxii, and Beijerinckia mobilis exhibited minimal TMPD oxidation capabilities (rates equivalent to the TMPD autooxidation reaction), and these extracts also possessed very active SODs but only of the Mn metallotype.
定量 N,N,N',N'-四甲基对苯二胺（TMPD）氧化酶和超氧化物歧化酶（SOD）分析在固氮菌科的代表性生物上进行。固氮菌属、棕色固氮菌、帕斯固氮菌和胶固氮菌表现出高的定量 TMPD 氧化酶活性，其提取物具有非常活跃且电泳均一（单凝胶带）的 Fe 型 SOD。巨细胞固氮菌的提取物具有类似的单 Fe 型 SOD，并且其细胞没有 TMPD 依赖性细胞色素氧化酶活性。印度拜叶林克氏菌、德氏拜叶林克氏菌和拜叶林克氏移动杆菌的固氮细胞表现出最小的 TMPD 氧化能力（速率相当于 TMPD 自氧化反应），这些提取物也具有非常活跃的 SOD，但仅为 Mn 金属型。
