在乳酸乳球菌 ML3 上增殖和纯化噬菌体 643 的方法。

Method for Growth and Purification of Bacteriophage 643 on Streptococcus lactis ML3.

机构信息

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Appl Environ Microbiol. 1984 Jul;48(1):242-4. doi: 10.1128/aem.48.1.242-244.1984.

DOI:10.1128/aem.48.1.242-244.1984

PMID:16346595

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240388/

Abstract

The yield of bacteriophage 643 was increased by infecting cultures of Streptococcus lactis ML3 in late-log phase growth, harvesting the infected cells, and suspending them in fresh, phosphate-buffered minimal medium. The cells lysed after this treatment and produced high titers of bacteriophage. The phage particles were dissociated from debris by 2 M NaCl and purified by differential and CsCl band centrifugation.

摘要

通过感染处于对数末期生长的乳链球菌 ML3 培养物，收获感染的细胞，并将其悬浮在新鲜的磷酸盐缓冲的最小培养基中，可以提高噬菌体 643 的产量。经过这种处理后，细胞裂解并产生高滴度的噬菌体。噬菌体颗粒通过 2 M NaCl 从碎片中解离出来，并通过差速和 CsCl 带离心进行纯化。