Lopez R, Ronda C, Tomasz A, Portoles A
J Virol. 1977 Oct;24(1):201-10. doi: 10.1128/JVI.24.1.201-210.1977.
We describe the purification and properties of Dp-1, a bacteriophage isolated from Diplococcus pneumoniae. The phage was sensitive to the organic solvents deoxycholate and Sarkosyl, and its infectivity was reduced by treatment with phospholipase C. Electron microscopy indicated the presence of a double-layered coat around the phage particles. Purified phage preparations contained lipid amounting to about 8.5% of the dry weight of the phage, and thin-layer chromatography resolved the lipids into four components. The phage had a buoyant density in CsCl of 1.47 g/cm3, and a sedimentation constant in 0.1 M NaCl of 313S. Analysis in acrylamide gel electrophoresis indicated the presence of three major proteins. Dp-1 DNA shows a density of 1.681 g/cm3. Neutralizing antisera against the phage have a low potency (K less than 120/min).
我们描述了从肺炎双球菌中分离出的噬菌体Dp-1的纯化方法及其特性。该噬菌体对脱氧胆酸盐和十二烷基肌氨酸钠等有机溶剂敏感，用磷脂酶C处理会降低其感染性。电子显微镜检查表明噬菌体颗粒周围存在双层衣壳。纯化的噬菌体制剂含有的脂质约占噬菌体干重的8.5%，薄层色谱法将脂质分离为四个组分。该噬菌体在CsCl中的浮力密度为1.47 g/cm3，在0.1 M NaCl中的沉降常数为313S。丙烯酰胺凝胶电泳分析表明存在三种主要蛋白质。Dp-1 DNA的密度为1.681 g/cm3。针对该噬菌体的中和抗血清效价较低（K小于120/分钟）。
