优化美洲鲶卵巢细胞和美洲鲶病毒 DNA 的生产条件。
Optimization of conditions for production of channel catfish ovary cells and channel catfish virus DNA.
机构信息
Department of Biochemistry, Mississippi State University, Mississippi State, Mississippi 39762.
出版信息
Appl Environ Microbiol. 1985 May;49(5):1025-8. doi: 10.1128/aem.49.5.1025-1028.1985.
Medium supplements were examined for their effect on the growth of channel catfish ovary cells. It was found that the usual serum supplement of 10% fetal calf serum could be successfully replaced with a combination of 5% fetal calf serum and a mixture of insulin, transferrin, and selenous acid. It was also found that these cells could be grown in a more efficient manner on microcarrier beads. This type of culture produced 14 times the number of cells per milliliter of total medium used compared with the usual tissue culture flasks used for cell growth. The microcarrier system also provided for greater production efficiency of DNA from channel catfish virus, a virus that infects this cell line.
研究了中量元素对牙鲆卵巢细胞生长的影响。结果发现，通常使用的 10%胎牛血清的血清补充物可以成功地被 5%胎牛血清和胰岛素、转铁蛋白和亚硒酸的混合物代替。还发现，这些细胞可以在微载体珠上以更有效的方式生长。与通常用于细胞生长的组织培养瓶相比，这种培养方式每毫升总培养基中产生的细胞数量增加了 14 倍。微载体系统还提高了感染该细胞系的牙鲆病毒的 DNA 生产效率。
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