Department of Oceanography, University of Hawaii, Honolulu, Hawaii, 96822, and Hawaii Institute of Marine Biology, Coconut Island, Kaneohe, Hawaii, 96744.
Appl Environ Microbiol. 1986 Oct;52(4):866-74. doi: 10.1128/aem.52.4.866-874.1986.
A method is presented for determining both the average turnover rate and the standard deviation of the average turnover rate of the adenine nucleotide (AN) pool within a population of microorganisms. The method requires the calculation of the initial slope and curvature of a plot of AN specific activity versus time following the introduction of [H]adenine. An analysis of noise-corrupted data indicated that the method is capable of detecting a lack of uniformity in the turnover rate when the coefficient of variation of the turnover rate exceeds 39%. An analysis of field data revealed a significant lack of uniformity in the turnover rates of microbial communities in a marine sediment sample and freshwater pond but no significant nonuniformity in the turnover rates of microbial communities in a seawater sample and in a second freshwater pond. Although the method has been applied only to the analysis of AN turnover rates, it is applicable to any intracellular pool for which a suitable radioactive precursor exists.
提出了一种方法，用于确定微生物群体中腺嘌呤核苷酸（AN）池的平均周转率和平均周转率的标准偏差。该方法需要在引入[H]腺嘌呤后，根据 AN 比活度与时间的关系图计算初始斜率和曲率。对噪声污染数据的分析表明，当周转率的变异系数超过 39%时，该方法能够检测到周转率的不均匀性。对现场数据的分析表明，在海洋沉积物样品和淡水池塘中的微生物群落的周转率存在显著的不均匀性，但在海水样品和第二个淡水池塘中的微生物群落的周转率没有显著的不均匀性。尽管该方法仅应用于 AN 周转率的分析，但它适用于任何存在合适放射性前体的细胞内池。
