从粘细菌 Cellvibrio mixtus 中克隆出编码纤维素酶、几丁质酶、淀粉酶和果胶酶的基因簇。
Cloning of a Gene Cluster from Cellvibrio mixtus which Codes for Cellulase, Chitinase, Amylase, and Pectinase.
机构信息
Department of Microbiology, University of Queensland, St. Lucia, 4067, Queensland, Australia.
出版信息
Appl Environ Microbiol. 1986 Dec;52(6):1362-7. doi: 10.1128/aem.52.6.1362-1367.1986.
Abstract
The soil isolate Cellvibrio mixtus UQM2294 degraded a variety of polysaccharides including microcrystalline cellulose. Among 6,000 cosmid clones carrying C. mixtus DNA, constructed in Escherichia coli with pHC79, 50 expressed the ability to degrade one or more of the following substrates: carboxymethyl cellulose, chitin, pectin (polygalacturonic acid), cellobiose, and starch. These degradative genes are encoded in a single 94.1-kilobase segment of the C. mixtus genome; a preliminary order of the genes is starch hydrolysis, esculin hydrolysis, cellobiose utilization, chitin hydrolysis, carboxymethyl cellulose hydrolysis, and polygalacturonic acid hydrolysis. A restriction endonuclease cleavage map was constructed, and the genes for starch, carboxymethyl cellulose, cellobiose, chitin, and pectin hydrolysis were subcloned.
摘要
土壤分离菌混合纤毛虫 UQM2294 可降解多种多糖，包括微晶纤维素。在利用 pHC79 构建的大肠杆菌中，携带混合纤毛虫 DNA 的 6000 个 cosmid 克隆中，有 50 个能够降解以下一种或多种底物：羧甲基纤维素、几丁质、果胶（多聚半乳糖醛酸）、纤维二糖和淀粉。这些降解基因编码在混合纤毛虫基因组的一个 94.1 千碱基片段中；这些基因的初步顺序是淀粉水解、七叶灵水解、纤维二糖利用、几丁质水解、羧甲基纤维素水解和聚半乳糖醛酸水解。构建了一个限制内切酶图谱，并对淀粉、羧甲基纤维素、纤维二糖、几丁质和果胶水解基因进行了亚克隆。
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