Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, and Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1988 May;54(5):1085-90. doi: 10.1128/aem.54.5.1085-1090.1988.
Six new xylanolytic bacterial strains have been isolated from a Napier grass-fed anaerobic digester. These strains were identified as Butyrivibrio fibrisolvens and were similar in many respects to ruminal isolates described previously. The new isolates exhibited a high degree of DNA homology with several ruminal strains of B. fibrisolvens. Xylan or xylose was required to induce the production of enzymes for xylan degradation, xylanase and xylosidase. Production of these enzymes was repressed in the presence of glucose. Xylanase activity was predominantly extracellular, while that of xylosidases was cell associated. The new isolates of B. fibrisolvens grew well in defined medium containing xylan as the sole carbon source and did not produce obvious slime or capsular layers. These strains may be useful for future genetic investigations.
从象草喂养的厌氧消化器中分离到 6 株新的木聚糖分解细菌菌株。这些菌株被鉴定为纤维丁酸弧菌，在许多方面与先前描述的瘤胃分离物相似。新的分离物与几种瘤胃纤维丁酸弧菌菌株具有高度的 DNA 同源性。需要木聚糖或木糖来诱导木聚糖降解酶，即木聚糖酶和木糖苷酶的产生。在葡萄糖存在的情况下，这些酶的产生受到抑制。木聚糖酶活性主要在细胞外，而木糖苷酶活性与细胞相关。纤维丁酸弧菌的新分离株在含有木聚糖作为唯一碳源的限定培养基中生长良好，并且不产生明显的粘液或荚膜层。这些菌株可能对未来的遗传研究有用。
