• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Identification of the Butyrivibrio fibrisolvens xylosidase gene (xylB) coding region and its expression in Escherichia coli.

作者信息

Sewell G W, Utt E A, Hespell R B, Mackenzie K F, Ingram L O

机构信息

Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.

出版信息

Appl Environ Microbiol. 1989 Feb;55(2):306-11. doi: 10.1128/aem.55.2.306-311.1989.

DOI:10.1128/aem.55.2.306-311.1989
PMID:2497707
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184106/
Abstract

The gene encoding the principal Butyrivibrio fibrisolvens xylosidase (xylB) has been cloned and expressed in Escherichia coli under the control of the lac promoter. The coding region for this gene was localized within a 3.2-kilobase B. fibrisolvens DNA fragment in pUC18. A new protein band was observed in recombinant E. coli containing xylB. This protein (approximately 60,000 molecular weight) was presumed to be the xylosidase monomer. The optimal pH (5.5) and substrate range for the recombinant and native xylosidases appeared identical. Both enzymes hydrolyzed xylo-oligosaccharides with chain lengths of 2 to 5 and both were inactive on xylan.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/08c196383ff1/aem00095-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/c7ae7618780e/aem00095-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/e9c190a6a977/aem00095-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/484b7851f022/aem00095-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/08c196383ff1/aem00095-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/c7ae7618780e/aem00095-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/e9c190a6a977/aem00095-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/484b7851f022/aem00095-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3256/184106/08c196383ff1/aem00095-0050-a.jpg

相似文献

1
Identification of the Butyrivibrio fibrisolvens xylosidase gene (xylB) coding region and its expression in Escherichia coli.
Appl Environ Microbiol. 1989 Feb;55(2):306-11. doi: 10.1128/aem.55.2.306-311.1989.
2
Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: a beta-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity.嗜热厌氧菌JW/SL YS485中两个基因的分离、分析及表达：一种β-木糖苷酶和一种具有头孢菌素C脱乙酰酶活性的新型乙酰木聚糖酯酶
J Bacteriol. 1997 Sep;179(17):5436-41. doi: 10.1128/jb.179.17.5436-5441.1997.
3
Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities.编码具有β-D-木糖苷酶和α-L-阿拉伯呋喃糖苷酶活性的新型双功能蛋白的溶纤维丁酸弧菌xylB基因的测序与表达
Appl Environ Microbiol. 1991 Apr;57(4):1227-34. doi: 10.1128/aem.57.4.1227-1234.1991.
4
Cloning, sequencing, and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens.来自厌氧瘤胃细菌溶纤维丁酸弧菌的木聚糖酶基因的克隆、测序及表达
J Bacteriol. 1990 Aug;172(8):4247-54. doi: 10.1128/jb.172.8.4247-4254.1990.
5
The genes for three xylan-degrading activities from Bacteroides ovatus are clustered in a 3.8-kilobase region.卵形拟杆菌三种木聚糖降解活性的基因聚集在一个3.8千碱基的区域。
J Bacteriol. 1990 May;172(5):2408-12. doi: 10.1128/jb.172.5.2408-2412.1990.
6
Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum".嗜热菌“解糖热纤梭菌”中木聚糖降解酶编码基因的克隆、序列分析及表达
Appl Environ Microbiol. 1990 Apr;56(4):1017-24. doi: 10.1128/aem.56.4.1017-1024.1990.
7
Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus.
Gene. 2000 Apr 18;247(1-2):137-43. doi: 10.1016/s0378-1119(00)00106-2.
8
Purification and properties of an aryl beta-xylosidase from a cellulolytic extreme thermophile expressed in Escherichia coli.从在大肠杆菌中表达的纤维素分解极端嗜热菌中纯化芳基β-木糖苷酶及其性质研究
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):645-50. doi: 10.1042/bj2730645.
9
Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.短小芽孢杆菌两个β-木糖苷酶基因的分离及其基因产物比较
Eur J Biochem. 1984 Jan 16;138(2):267-73. doi: 10.1111/j.1432-1033.1984.tb07911.x.
10
Identification and characterization of clustered genes for thermostable xylan-degrading enzymes, beta-xylosidase and xylanase, of Bacillus stearothermophilus 21.嗜热脂肪芽孢杆菌21中热稳定木聚糖降解酶、β-木糖苷酶和木聚糖酶的成簇基因的鉴定与表征
Appl Environ Microbiol. 1994 Jul;60(7):2252-8. doi: 10.1128/aem.60.7.2252-2258.1994.

引用本文的文献

1
Molecular cloning and comparative sequence analysis of fungal β-Xylosidases.真菌β-木糖苷酶的分子克隆及比较序列分析
AMB Express. 2016 Dec;6(1):30. doi: 10.1186/s13568-016-0202-3. Epub 2016 Apr 14.
2
Purification and Characterization of an alpha-l-Arabinofuranosidase from Butyrivibrio fibrisolvens GS113.从纤维丁酸弧菌 GS113 中纯化和鉴定 α-l-阿拉伯呋喃糖苷酶。
Appl Environ Microbiol. 1992 Apr;58(4):1082-8. doi: 10.1128/aem.58.4.1082-1088.1992.
3
The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl beta-D-xylosides and unsubstituted xylans.

本文引用的文献

1
Rate of isolated hemicellulose degradation and utilization by pure cultures of rumen bacteria.瘤胃细菌纯培养物对分离出的半纤维素的降解和利用速率。
Appl Microbiol. 1967 Sep;15(5):987-93. doi: 10.1128/am.15.5.987-993.1967.
2
Isolation and Characterization of Xylan-Degrading Strains of Butyrivibrio fibrisolvens from a Napier Grass-Fed Anaerobic Digester.从饲喂象草的厌氧消化器中分离和鉴定产木聚糖酶丁酸纤维梭菌菌株。
Appl Environ Microbiol. 1988 May;54(5):1085-90. doi: 10.1128/aem.54.5.1085-1090.1988.
3
Isolation and Some Properties of a beta-d-Xylosidase from Clostridium acetobutylicum ATCC 824.
艾默生篮状菌的木聚糖降解酶系统：对芳基β-D-木糖苷和未取代木聚糖具有活性的新型酶
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):515-23. doi: 10.1042/bj2900515.
4
Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger.黑曲霉主要α-L-阿拉伯呋喃糖苷酶（ABF B）编码基因abfB的克隆与特性分析
Curr Genet. 1993 Dec;24(6):525-32. doi: 10.1007/BF00351717.
5
Degradation and utilization by Butyrivibrio fibrisolvens H17c of xylans with different chemical and physical properties.纤维溶解丁酸弧菌H17c对具有不同化学和物理性质的木聚糖的降解与利用
Appl Environ Microbiol. 1995 Aug;61(8):3042-50. doi: 10.1128/aem.61.8.3042-3050.1995.
6
Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.瘤胃拟杆菌B(1)4内切葡聚糖酶基因的克隆与测序
J Bacteriol. 1990 Jul;172(7):3620-30. doi: 10.1128/jb.172.7.3620-3630.1990.
7
Cloning, sequencing, and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens.来自厌氧瘤胃细菌溶纤维丁酸弧菌的木聚糖酶基因的克隆、测序及表达
J Bacteriol. 1990 Aug;172(8):4247-54. doi: 10.1128/jb.172.8.4247-4254.1990.
8
Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities.编码具有β-D-木糖苷酶和α-L-阿拉伯呋喃糖苷酶活性的新型双功能蛋白的溶纤维丁酸弧菌xylB基因的测序与表达
Appl Environ Microbiol. 1991 Apr;57(4):1227-34. doi: 10.1128/aem.57.4.1227-1234.1991.
9
Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains.Tn916、Tn916 delta E和pAM beta 1从粪肠球菌向溶纤维丁酸弧菌菌株的接合转移。
Appl Environ Microbiol. 1991 Sep;57(9):2703-9. doi: 10.1128/aem.57.9.2703-2709.1991.
从丙酮丁醇梭菌 ATCC 824 中分离得到β-D-木糖苷酶及其性质
Appl Environ Microbiol. 1987 Apr;53(4):651-4. doi: 10.1128/aem.53.4.651-654.1987.
4
The pentosanases of some rumen bacteria.一些瘤胃细菌的戊聚糖酶
Biochem J. 1960 Jan;74(1):173-80. doi: 10.1042/bj0740173.
5
N-(1-naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process.盐酸 N-(1-萘基)乙二胺作为一种新试剂，通过数学校准过程对薄层板上的糖进行纳摩尔定量分析。
Anal Biochem. 1980 Aug;106(2):291-5. doi: 10.1016/0003-2697(80)90523-0.
6
Microbial ecology and activities in the rumen: Part II.瘤胃中的微生物生态学与活性：第二部分。
Crit Rev Microbiol. 1982 May;9(4):253-320. doi: 10.3109/10408418209104492.
7
Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.短小芽孢杆菌两个β-木糖苷酶基因的分离及其基因产物比较
Eur J Biochem. 1984 Jan 16;138(2):267-73. doi: 10.1111/j.1432-1033.1984.tb07911.x.
8
Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli.短小芽孢杆菌木聚糖降解基因的分子克隆及其在大肠杆菌中的表达。
Mol Gen Genet. 1983;192(3):335-41. doi: 10.1007/BF00392172.
9
Pectin-fermenting bacteria isolated from the bovine rumen.从牛瘤胃中分离出的果胶发酵细菌。
J Bacteriol. 1969 Jul;99(1):189-96. doi: 10.1128/jb.99.1.189-196.1969.
10
Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.麻风分枝杆菌DNA的分子分析及基因组文库的构建。
J Bacteriol. 1985 Mar;161(3):1093-102. doi: 10.1128/jb.161.3.1093-1102.1985.