一种简单、快速的方法，用于计数地下水中总活菌和代谢活跃菌。
A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater.
机构信息
Department of Biology, Virginia Polytechnic Institute & State University, Blacksburg, Virginia 24061.
出版信息
Appl Environ Microbiol. 1988 Jun;54(6):1630-1. doi: 10.1128/aem.54.6.1630-1631.1988.
Abstract
We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4',6'-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.
摘要
我们在此报告一种新的染色程序，它同时使用 2-(对碘苯基)-3-p-(硝苯基)-5 苯基四唑氯化物的酶促脱氢作用将其转化为粉红色的细胞内甲臜，以及 DNA 特异性荧光染料 4',6'-二脒基-2-苯基吲哚。将这种染色程序应用于通过过滤转移-冷冻技术浓缩在滤器上然后转移到显微镜载玻片上的细胞，已被证明对于统计准确地计数地下水中总存活和代谢活跃的细胞非常有价值。
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