Hobbie J E, Daley R J, Jasper S
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Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter. Although cellulose filters also retain all of the bacteria, many are trapped inside the filter where they cannot be counted. Before use, the Nuclepore filters must be dyed with irgalan black to eliminate autofluorescence. Direct counts of bacteria in lake and ocean waters are twice as high with Nuclepore filters as with cellulose filters.
聚碳酸酯核孔滤膜在直接计数细菌方面比纤维素滤膜更好，因为它们具有均匀的孔径和一个平坦的表面，能将所有细菌保留在滤膜顶部。虽然纤维素滤膜也能保留所有细菌，但许多细菌被困在滤膜内部，无法进行计数。在使用前，核孔滤膜必须用伊加仑黑染色以消除自发荧光。使用核孔滤膜对湖泊和海洋水体中的细菌进行直接计数，其结果是使用纤维素滤膜时的两倍。
