Laboratory of Microbial Chemistry, Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, and Infectious Diseases, Veterans' Medical Center, Albuquerque, New Mexico 87108.
Appl Environ Microbiol. 1988 Jul;54(7):1831-7. doi: 10.1128/aem.54.7.1831-1837.1988.
The cell surface of Azospirillum brasilense was probed by using fluorescein isothiocyanate (FITC)-labeled lectins, with binding determined by fluorescence-activated flow cytometry. Cells from nitrogen-fixing or ammonium-assimilating cultures reacted similarly to FITC-labeled lectins, with lectin binding in the following order: Griffonia simplicifolia II agglutinin > Griffonia simplicifolia I agglutinin > Triticum vulgaris agglutinin > Glycine max agglutinin > Canavalia ensiformis agglutinin > Limax flavus agglutinin > Lotus tetragonolobus agglutinin. The fluorescence intensity of cells labeled with FITC-labeled G. simplicifolia I, C. ensiformis, T. vulgaris, and G. max agglutinins was influenced by lectin concentration. Flow cytometry measurements of lectin binding to cells was consistent with measurements of agglutination resulting from lectin-cell interaction. Capsules surrounding nitrogen-fixing and ammonium-assimilating cells were readily demonstrated by light and transmission electron microscopies.
采用荧光素异硫氰酸酯（FITC）标记的凝集素探测巴西固氮螺菌的细胞表面，通过荧光激活流式细胞术测定结合情况。固氮或铵同化培养物的细胞对 FITC 标记的凝集素反应相似，凝集素结合顺序如下：相思豆 II 凝集素>相思豆 I 凝集素>普通小麦凝集素>大豆凝集素>刀豆凝集素>黄粉鹿花虫凝集素>四棱豆凝集素。用 FITC 标记的 G. simplicifolia I、C. ensiformis、T. vulgaris 和 G. max 凝集素标记的细胞的荧光强度受凝集素浓度的影响。凝集素与细胞结合的流式细胞术测量与凝集素-细胞相互作用产生的凝集测量一致。固氮和铵同化细胞周围的荚膜通过光镜和透射电子显微镜很容易观察到。
