从氧化木聚糖醋酸杆菌亚种 MI-4 生产 d-氨基酰化酶。
Production of d-Aminoacylase from Alcaligenes denitrificans subsp. xylosoxydans MI-4.
机构信息
Department of Environmental Chemistry and Engineering, Faculty of Engineering, Oita University, Oita 870-11, Japan.
出版信息
Appl Environ Microbiol. 1988 Nov;54(11):2767-70. doi: 10.1128/aem.54.11.2767-2770.1988.
Abstract
A bacterial strain that produces d-aminoacylase was isolated from soil and identified as Alcaligenes denitrificans subsp. xylosoxydans MI-4. l-Aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-Aminoacylase was inducibly produced. N-Acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. Enzymatic resolution of N-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was approximately 100/7, suggesting that resolution with crude enzymes may become possible by removing small amounts of the contaminated l-form with l-amino acid oxidase.
摘要
从土壤中分离到一株产 d-氨基酰化酶的细菌，并鉴定为黄杆菌属（Alcaligenes）的木质糖氧化亚硝化亚种（subsp. xylosoxydans）MI-4。该菌的 l-氨基酰化酶活仅为 d-氨基酰化酶活的 1%~2%。d-氨基酰化酶是可诱导产生的。N-乙酰-dl-亮氨酸是最佳诱导物，且 d-异构体具有诱导酶产生的能力。用粗酶进行 N-乙酰-dl-蛋氨酸的酶法拆分，拆分得到的蛋氨酸的 d/l 比约为 100/7，表明通过用 l-氨基酸氧化酶去除少量污染的 l-型，粗酶拆分可能成为可能。
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