嗜热梭菌无孢子形成突变体增强纤维素发酵。
Enhanced Cellulose Fermentation by an Asporogenous and Ethanol-Tolerant Mutant of Clostridium thermocellum.
机构信息
Unité de Physiologie Cellulaire et URA 209 CNRS, Département des Biotechnologies, Institut Pasteur, 28, rue du Dr Roux, 75724 Paris Cedex 15, France.
出版信息
Appl Environ Microbiol. 1989 Jan;55(1):207-11. doi: 10.1128/aem.55.1.207-211.1989.
Abstract
A mutant of Clostridium thermocellum isolated after UV mutagenesis and selection for resistance to fluoropyruvate was found to be asporogenous and ethanol tolerant. The mutant was also an ethanol hyperproducer, able to ferment 63 g of cellulose into 14.5 g of ethanol per liter of medium. The ratio of ethanol to total organic acids produced by the mutant was increased, and H(2) production was decreased. Culture conditions were optimized for ethanol production by the new strain.
摘要
一株经紫外线诱变和氟丙酮酸抗性选择得到的热纤梭菌突变株具有不产孢子和耐乙醇的特性。该突变株也是乙醇的超产菌，能够将 63 克纤维素发酵为 14.5 克乙醇/升培养基。突变株产生的乙醇与总有机酸的比例增加，H2产量降低。对新菌株的乙醇生产条件进行了优化。
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